Abstract
A cellulase (CMCase) gene of Cellulomonas uda CB4 was introduced into Zymomonas mobilis NRRL B-14023 on pZA22, a cloning vector for Zymomonas, by conjugal transfer. Z. mobilis carrying this gene synthesized cellulase immunologically identical with that of C. uda CB4, by transcriptional read-through from the promotor of the chloramphenicol resistance (chloramphenicol acetyltransferase) gene within pZA22. A strong promotor containing a translation initiation signal was obtained from Z. mobilis NRRL B-14023 chromosomal DNA. By gene fusion between this Zymomonas promotor fragment and the truncated cellulase gene, the activity of cellulase synthesized in Z. mobilis reached 0.78 units per ml culture, six-fold higher than that by transcriptional read-through from the promotor of the chloramphenicol resistance gene.
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