Expression of β-Defensin-1 and 2 in HPV-Induced Epithelial Lesions

Abstract

Abstract α, β, θ defensins represent a family of small antimicrobial peptides expressed predominantly by a series of cells, including neutrophils, monocytes-macrophages and epithelial cells that are involved in defense mechanisms against viral infections. β-defensins are the most widespread in this family being encountered in oral, digestive, urogenital mucosa and cutaneous lesions. β-defensins directly inactivate certain viruses, including the human papillomavirus(HPV) suppressing viral replication by altering target cells. Considering these aspects, we investigated the immunohistochemical expression of β-defensin-1 and 2 in HPV-induced epithelial lesions. For this study, tumoral and normal mucosal tissue fragments were collected from 10 patients aged between 31-60years, with previously confirmed HPV infection, diagnosed clinically and histopathologically with cervical carcinoma. Patients did not receive any chemotherapy or radiotherapy before the biopsy procedure. The tissue fragments were processed by the standard immunohistochemistry technique using anti-β-defensin-1 and 2 antibodies(Bioss Antibodies). The samples examination revealed weak positive(+) membrane, cytoplasmic and nuclear IR for hβD-1 in basal layer of normal cervical mucosa and moderate positive(++) membrane and cytoplasmic IR in squamous epithelium. For dysplastic HPV-associated tissues we highlighted a nuclear moderate positive(++) IR.For hβD-2, IR in basal layer of the normal mucosa was lower(+) compared with dysplastic cells IR and showed a strong expression(+++) at membrane, cytoplasmic and nuclear level in koilocytes of patients with HPV-associated dysplasia. It was also observed a moderate positive (++) IR in basal layer of dysplastic cells of patients without HPV. The obtained results are in agreement with some literature data, which highlighted the fact that hβD-1 and hβD-2 are very important components of the molecular pattern in HPV-induced lesions.