Expression level of lipoprotein lipase in patients with chronic lymphocytic leukemia and its correlation with other prognostic factors

Abstract

18000 Background: Chronic lymphocytic leukemia (CLL) is the most common adult form of leukemia in the Western world, however, infrequent in the Eastern. It shows a remarkable heterogeneity, with some patients having an almost normal lifespan, others surviving only several years after diagnosis despite intensive therapy. A series of recent microarray studies have identified a number of other potential prognostic factors that have not yet been validated for their clinical importance. One of the genes most closely related to IgVH mutational status is lipoprotein lipase (LPL). Methods: To investigate LPL expression level in Chinese patients with CLL and its correlation with other prognostic factors, including IgVH mutation status, Binet stages, ZAP-70 protein and CD38 expression level, semiquantitative RT-PCR was used to detect LPL expression in peripheral blood samples of 58 Chinese patients with CLL. IgVH somatic mutational status was detected by multiplex PCR and sequencing of purified PCR amplification pr...