Expression level and significance of NGF, TGF-β1, estradiol, testosterone in serum and expressed prostatic secretion of patients with category III prostatitis

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Objective To investigate the level and clinical significance of nerve growth factor （ NGF） , transforming growth factor （ TGF ）-β1 , estradiol （ E2 ） and testosterone （ T ） in serum and ex-pressed prostatic secretion （EPS） of patients with category Ⅲprostatitis. Methods From August 2011 to January 2012, 64 patients with （chronic prostatitis/chronic pelvic pain syndrome , CP/CPPS） and 20 health people were enrolled in this study.In CP/CPPS group, the age of patients ranged from 18 to 56 years, mean （36.6±9.3） years.The history of CP/CPPS ranged from 3 months to 6 years, mean 2 years.All patients were asked to complete NIH-CPSI questionnaires with CP/CPPS, including group ⅢA 35 cases and groupⅢB 29 cases.The age of healthy controls ranged from 25 to 41 years.The average healthy control age was （33.1±3.9） years.EPS and serum samples from CP/CPPS and control group were collected and frozen . NGF, TGF-β1 , E2 and T level in EPS and serum were measured by ELISA and radioimmunoassay and com -pared in each group. Results The mean E2, E2/T, TGF-β1 level in serum of patients with CP/CPPS were （175.7±82.4） pmol/L, （7.9±6.7）, （2 216.2±581.6） ng/L, which were higher than that in healthy controls, （131.7±49.4） pmol/L, （4.6±2.4）, （1 599.8±469.5） ng/L.The mean T level in CP/CPPS pa-tients′serum was （24.7±8.9） nmol/L, which was lower than that in controls （29.2±7.0） nmol/L.The E2/T （34.5±29.8）, TGF-β1（6 859.3±5 229.4 ng/L）, NGF （467.0±164.3 ng/L） levels in EPS of CP/CPPS patients were higher than that in controls （16.5±13.8）, （1 774.1±1 304.3） ng/L, （310.8±106.6） ng/L. The TGF-β1 level in EPS of CP/CPPS patients showed the positive correlation ship with urination symptom score （6.1±2.4） （r=0.641, P〈0.05）.The NGF level in EPS of CP/CPPS patients also showed the positive correlation ship with pain score （7.6±2.6） （r=0.497, P〈0.05）.E2/T,TGF-β1 levels in serum and E2/T, TGF-β1,NGF levels in EPS of group ⅢA were （7.1±4.6）, （2131.5±412.0）ng/L and （31.5±22.3）, （7 667.1±5 652.4）ng/L, （440.6±134.3）ng/L, which were significantly higher than those in healthy con-trol （P〈0.05）.E2/T, TGF-β1 levels in serum and E2/T, TGF-β1, NGF levels in EPS of group ⅢB were （8.9±8.5）, （2 340.5±728.2） ng/L and （38.2±37.1）, （5 884.4±4 574.3） ng/L, （498.9±192.1） ng/L, which were also higher than those in healthy control （ P〈0.05） . Conclusions Hormonal imbalance in es-tradiol and testosterone with TGF-β1 , NGF higher levels in EPS is closely related with pathogenesis and clin-ical symptom of category III chronic nonbacterial prostatitis . Key words: Prostatitis; Nerve growth factor; Transforming growth factor; Sex hormone