Expression in vivo and in vitro of the major structural protein (VP73) of African swine fever virus.

Abstract

The VP73 protein was produced by in vitro transcription and translation from the Xho I-Bam HI fragment located between the Cla I-N and Cla I-H fragments of the viral genome. This DNA fragment encodes a late mRNA of about 2.6 kb detected in infected MS monkey and BHK hamster cells. The transcript was initiated at a site within two bases upstream of the translation initiation codon. The in vitro synthesized polypeptide shows the same molecular weight as the in vivo synthesized polypeptide, suggesting that VP73 has no post-translational modification. There are two internal AUG initiation codons for in vitro translation, one of which is functional in vivo, as well as a possible GUG initiator codon detected by expression of the protein in E. coli cells.