Abstract
Fusions between regions upstream of eryAI and the aph reporter gene were studied. In high copy number plasmids, DNA extending 262 bp upstream of the EryAI translation start sufficed for full kanamycin/neomycin phosphotransferase (APH) expression in Streptomyces lividans. Low copy number constructs gave similar APH activities in Saccharopolyspora erythraea and S. lividans, in accord with the idea that there is no pathway-specific regulatory gene in this system. © Rapid Science Ltd. 1998
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