Expression differences of N-acetyl amino galactosyl transferase-14 in the different levels of malignant glioma and the correlation of malignant degree with glial tumors

Abstract

Objective To explore the expression differences of N-acetyl amino galactosyl transferase-14 (GalNAc-T14) in normal brain tissue and different levels of malignant gliomas and to observe the relationship between the expression of GalNAc-T14 with the differentiation and malignant degree of gliomas.Methods By real-time quantitative polymerase chain reaction detecting system (QPCR) and immunohistochemical staining methods,the expression of GalNAc-T14 was detected in 11 cases of Ⅱ grade glioma tissue samples,14 cases of Ⅲ grade glioma tissue specimens,9 cases of Ⅳ grade glioma tissue samples and 16 specimens of normal brain tissue.Results The average optical density values of GalNAc-T14 in normal brain tissue specimens and the specimens of Ⅱ,Ⅲ and Ⅳ grade glioma cells were 0.956 4 ±0.079 1,0.525 6 ±0.064 7,0.298 9 ±0.080 8,and0.151 4 ±0.062 4 respectively by QPCR.Single factor analysis of variance (One-way ANOVA) and LSD,SNK-t analysis comparison showed the expression of GalNAc-T14 in human gliomas was significantly different from that in the normal brain tissue (P ＜ 0.05).The same significance was also found between grade Ⅱ and grade Ⅲ or grade Ⅳ (P ＜ 0.05).Immunohistochemical staining method revealed that the positive rate of the GalNAc-T14 expression was 68.7％ in normal brain tissue specimens and 63.6％,42.9％ and 33.3％ in glioma tissues of grade Ⅱ,Ⅲ and Ⅳ,and there was significant difference between glioma group and normal brain tissue groups (P ＜ 0.05).Different levels of brain glioma cells showed no obvious consistency.Conclusion The expression of GalNAc-T14 was significantly reduced in malignant glioma cells,and was closely related with disease progression. Key words: Glioma; N-acetyl amino galactosyl transferase-14; Apoptosis; O-glycosylation