Expression differences of Cathepsin B and Cathepsin D in primary gallbladder cancer, gallbladder adenomatous polyps and chronic cholecystitis

Abstract

Objective To investigate the expression of cathepsin B (CatB) and cathepsin D (CatD) in primary gallbladder carcinoma,adenomatoid polyp without malignant transformation,and chronic cholecystitis tissues and to assess their mutual correlation ships as well as the relationships between their expressions and clinical pathological features.Methods Detected the expressions of CatB and CatD in gallbladder tissues including 32 specimens of primary gallbladder carcinoma,10 specimens of adenomatoid polyp without malignant transformation and 10 specimens of chronic cholecystitis by immunohistochemical method.The expressions were assessed by semi-quantitative analysis.Results The positive rate of CatB,CatD and CCL20 in primary gallbladder carcinoma was 93.75％ (30/32) and 90.63％ (29/32) respectively,being significantly higher than those in adenomatoid polyp without malignant transformation (60％and 60％,P ＜0.05) and chronic cholecystitis groups (60％ and 50％ P ＜0.05).The expression levels of CatB and CatD were not significantly related to the clinical features including age and gender,or pathological features including grades of cell differentiation,pathological types and Nevin stages ( P ＞ 0.05 ).CatB and CatD had significantly positive correlations ( r =0.434,P ＜ 0.05 ).Conclusion ( 1 ) CatB and CatD CCL20 might serve as the histological marker of primary gallbladder carcinoma.(2) The expression levels of CatB and CatD in primary gallbladder carcinoma are not related to the clinical or pathological features.(3) There is positive correlation between the expression levels of CatB and CatD in primary gallbladder carcinoma. Key words: Primary gallbladder carcinoma; Cathepsin B; Cathepsin D; Immunohistochemistry