Abstract
Objective To investigate the expression characteristic of SEPT11 in mouse testis and its role in spermatogenesis. Methods Real-time PCR, Western blotting and immunofluorescence were applied to study the expression characteristics of gene SEPT11 in mouse testis at different postnatal week and from Sertoli cell-selected Ar knockout mice (SCARKO) and Ar knockout (ARKO) adult mice. Results The expression of SEPT11 was highest during postnatal 2 weeks, and then fall down. SEPTIN11 colocalized with mature sperm attached with Sertoli cells in the testis from mice at postnatal 6 weeks. The expression of SEPTIN11 in SCARKO and ARKO mice testis were much lower compared with the wild type (P<0.01), the mRNA level was significantly higher (P<0.01). SEPTIN11 localized to the tail of mature sperm collected from epididymis. Conclusion The new born mice had the highest expression level of SEPT11. SEPTIN11 localized to the tail of mature sperm. Knock-down of Ar increased the expression of SEPT11. Key words: SEPT11; Spermatogenesis; Testis; Mouse
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