Expression characteristics of annexin A2 in dermal papilla cells with aggregative behavior

Abstract

Objective To analyze the expression characteristics of annexin A2 in dermal papilla cells (DPCs) with aggregative behavior. Methods Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot were performed to measure the mRNA and protein expressions of annexin A2 respectively in DPCs with or without aggregative behavior. Results The mRNA expression level of annexin A2 was significantly higher in DPCs with aggregative behavior than in those without aggregative behavior (0.50 ± 0.15 vs. 0.35 ± 0.19, t= 8.26, P< 0.05). Western blot showed that annexin A2 had two isoforms, including one isoform with a relative molecular mass of 40 000 and the other one with a relative molecular mass of 36 000. The annexin A2 isoform with a relative molecular mass of 40 000 was highly expressed in both DPCs with aggregative behavior and those without aggregative behavior, while the other isoform was only expressed in DPCs with aggregative behavior. Conclusion Annexin A2 may be closely related to the aggregative growth of DPCs. Key words: Hair follicle; Annexin A2; Cell growth processes; Reverse transcriptase polymerase chain reaction; Blotting, Western; Dermal papilla cells