Expression change of CAT in lung of renal ischemia-reperfusion injury rats

Abstract

Objective To observe the expression of catalase (CAT) in the lung of renal ischemia-reperfusion injury rats and study its role in oxidative-stress response. Methods After the right kidney of rats was removed, the left renal artery was clipped with non-damage vascular clamp to create renal ischemia-reperfusion injury model.After 24 hours of reperfusion, the blood, kidney and lung were taken.The blood urea nitrogen (BUN) and serum creatinine (SCr) were detected with enzyme coupling rate method and picric acid method.The malondialdehyde (MDA) content in lung tissue was determined by thiobarbituric acid colorimetric method.The renal morphology change was observed by HE staining.The expressions of CAT mRNA and protein were evaluated by reverse transcription polymerase chain reaction and Western blot. Results The HE staining results showed that kidney was impaired in renal ischemia-reperfusion injury group.Compared with the control group, the BUN, SCr in serum and MDA in the lung of renal ischemia-reperfusion injury group were significantly increased (P<0.05 or P<0.01), the expressions of CAT mRNA and protein were enhanced (all P<0.01). Conclusions After renal ischemia-reperfusion injury, lung tissue has the peroxide damage.CAT may play an important role in inhibiting the oxidative-stress response in the lung of renal ischemia-reperfusion injury rats. Key words: Renal ischemia-reperfusion injury; Catalase; Malondialdehyde; Oxidative stress; Hydrogen peroxide