Expression and subcellular localization of HSPC117 in min pig tissues and the PK15 cell line.

Abstract

BACKGROUND: The human hematopoietic stem/progenitor cell 117 (HSPC117) protein is involved in many important biological processes.OBJECTIVE:This study was designed to identify the level of HSPC117 mRNA expression in 10 min pig tissue samples and HSPC117 subcellular localization in the PK15 cell line.METHODS: In this study, 10 tissue samples of min pigs were collected, and EGFP-HSPC117 vectors were constructed to express EGFP-HSPC117 fusion proteins in PK15 cells.RESULTS: HSPC117 mRNA was expressed in all of the tissue samples, although the levels of expression in fat and lung tissues were significantly lower than in other tissues ( 0.01). After generating and detecting the EGFP-HSPC117 fusion protein, fluorescence was found to be distributed throughout the cytoplasm and nucleus during interphase; however, the fluorescence was concentrated in the nuclear area in mitotic cells.CONCLUSIONS: These results indicate that the HSPC117 gene is expressed in many min pig tissues. The HSPC117 protein was distributed throughout the cells during interphase, but was concentrated in the nuclear area in mitotic cells.