Expression and significance of NF-κB and MIF in lung tissue of silicon-stained rats

Abstract

To investigate the distribution and expression of nuclear transcription factor(NF-κB) and macrophage migration inhibitory factor(MIF) in the lung tissue of rats exposed to silica. Atotal of sixty-four adult rats aged 10 to 12 weeks(cleaning grade) were randomly divided into control group and silica dust group(32 rats each). At day 3, 7, 14, and 28 after modeling, 8 rats were randomly sacrificed in each group. Immunohistochemistry was used to detect the distribution and expression of NF-B and MIF in lung tissue. The positive result of NF-κB p65 were localized in the nucleus and cytoplasm, and were mainly expressed in bronchial wall epithelial cells, macrophages, neutrophils, lung mesenchymal cells, and alveolar epithelial cells. The IOD value of NF-κB p65 in the dust-exposed group was compared with the control group at each time point, and the difference was statistically significant(P<0. 05). The result of MIF immunohistochemistry in rat lung tissue showed that: MIF-positive cells were mainly alveolar epithelial cells, mesenchymal macrophages and alveolar macrophages. Among them, positive expression was also found in part of the capillary endothelium, and in smooth muscle cells of the vessel wall There is also a small amount of expression. The MIF IOD value of the dust-exposed group was compared with the control group at each time point, and the difference was statistically significant(P<0. 05). Both NF-κB and MIF are positively expressed in the lung tissue of silica-exposed rats, which has certain reference significance in the early diagnosis and treatment of silicosis in the future.