Expression and significance of NADPH oxidase DUOX1 in hepatocellular carcinoma after tumor resection

Abstract

Objective To study the prognostic value of dual oxidase 1(DUOX1) gene in hepatocellular carcinoma(HCC). Methods Reverse transcription polymerase chain reaction (RT-PCR) analysis were used to detect the expression of DUOX1 mRNA in 7 HCC cell lines, one normal liver cell line, specimen of normal liver tissues in 30 cases and paired specimen of neoplastic with matched adjacent non-neoplastic liver tissues in 103 cases. The correlation of the DUOX1 mRNA expression in neoplastic tissues were analyzed with clinicopathological and prognostic factors. Results DUOX1 mRNA was expressed in MHCC-97H, MHCC-97L and BEL7402 cell lines, but not in HepG2, Hep3B, SMMC-7721, Chang liver and LO2 cell line. The expression of DUOX1 mRNA was not found in all 30 normal liver tissues. The positive rates of DUOX1 expression in neoplastic tissues was higher than in adjacent non-neoplastic tissues(53.4% vs 14.5%, P <0.01), respectively. Univariate analysis indicated that factors affecting overall survival were gender, age, tumor size, satellite nodule, tumor thrombus in the portal vein, TNM stage and DUOX1 expression status in neoplastic tissues. Multivariate analysis indicated that independent factors affecting overall survival were tumor size, tumor thrombus in the portal vein and DUOX1 expression status. Conclusions DUOX1 expression in HCC neoplastic tissues is an independent factor affecting the prognosis of patients with HCC after tumor resection. DUOX1 may play a role in liver carcinogenesis. Key words: NADPH oxidase; Hepatocellular Carcinoma; Prognosis; DUOX1