Expression and significance of MMP-9, MMP-12 and IP-10 in the two emphysema models

Abstract

Objective To make emphysema models by active immunization and cigarette smoking using guinea pigs,then observe the expression of MMP-9,MMP-12,IP-10 and the destruction of pulmonary tissue and myocardial tissue in each model,to explore the role of the MMP-9,MMP-12,IP-10in the emphysema which induced by active immunization.Methods The guinea pigs were randomly divided into three groups,involving active immunization group,smoking group and control group.The active immunization group used the method of subcutaneous injection of elastin at multiply points and the smoking group used the traditional method,while the control group performed nothing.To observe the morphosis of pulmonary tissue and myocardial tissue and the expression of MMP-9,MMP -12,IP-10 in the pulmonary tissue and myocardial tissue of every model were observed by immunohistochemical staining,Results The expression of MMP-9,MMP-12,IP -10 in the pulmonary tissue and myocardial tissue has both significantly increased in the active immunization group and the smoking group,compared with the control group ( P ＜0.05).The expression of MMP-9,MMP-12 in the pulmonary tissue in the active immunization group compared with the smoking group,and there is no obvious difference ( P ＞0.05).The expression of matrix MMP 9,MMP-12 in the myocardial tissue in the active immunization group,compared with the smoking group ( P ＜ 0.05).Conclusions Emphysema model of guinea pig can be established by active immunization.MMP-9,MMP-12,IP- 10 has important positive influence on emphysema which induced by active immunization. Key words: Emphysema; Autoimmunity; Matrix Metalloproteinase-9; Matrix Metalloproteinase-12; Interferon-inducible protein-10