Expression and significance of miR-10b in hepatocellular carcinoma tissue samples

Abstract

Objective To detect the expression of miR-10b in hepatocellular carcinoma （HI;G） tissue samples, and to explore its association with clinicopathologic features. Methods qRT-PCR analysis was used to detect expression of miR-10b in 58 HCC tissues （Among them, 32 cases were diagnosed with metastasis by pathological analysis, 26 cases without metastases at the time of resection） and 10 normal liver tissues. Statistical analysis was used to determine the association of miR-10b expression with clinicopathologic features and prognosis of patients. Results miR-10b was up-regulated both in metastasis-free and metastatic HCC tissues, but its up-regulated degree was much more significant in metastatic HCC tissues （P~0.05）. High miR-10b expression was strongly correlated with the metastasis （P〈0.01） and AJCC stage（P=0. 016）. But it was not correlated with age, gender, hepatitis B, cirrhosis, tumor size, degree of differentiation, and tumor number. Patients with high miR-10b expression had significantly poorer overall survival（P〈0.01）. High miR-10b expression was independent prognostic factors for overall survival （P=0. 016）. Conclusions The expression of miR- 10b is highly up-regulated in HCC tissues, especially in metastatic HCC tissues, indicating that high miR-10b expression may be involved in the process of metastasis and is expected to become a new prognosis reference of HCC. Key words: Micro RNA-10b; Hepatocellular carcinoma; Metastasisi