Abstract
目的本研究分析溶酶体相关4次跨膜蛋白B(lysosome-associated transmembrane protein 4β,LAPTM4B)-35和基质金属蛋白酶(matrix metalloproteinase,MMP)-9在胃癌中的表达及其与临床病理因素和预后的关系。 方法采用qRT-PCR法检测胃癌细胞株及正常胃黏膜细胞株中LAPTM4B-35和MMP-9 mRNA的表达,免疫组织化学分析胃癌石蜡标本LAPTM4B-35和MMP-9蛋白的表达,运用相关手段分析蛋白表达与临床相关性,Log-rank单因素分析和Cox多因素分析对预后进行研究。 结果LAPTM4B-35和MMP-9 mRNA在HGC-27、SGC7901和MGC803胃癌细胞株相对表达量明显高于GES-1胃黏膜细胞株;免疫组织化学结果显示LAPTM4B-35和MMP-9均在细胞质中表达,LAPTM4B-35阳性表达率为50.7%,MMP-9阳性表达率为36.8%;LAPTM4B-35的表达与MMP-9的表达呈正相关;相关分析显示Lauren's分型和肿瘤浸润T分期与LAPTM4B-35表达相关,肿瘤大小和淋巴结转移N分期与MMP-9表达密切相关,预后生存Cox多因素分析结果显示分化程度、淋巴结转移N分期、LAPTM4B-35表达与MMP-9表达是胃癌患者独立预后因素。 结论LAPTM4B-35和MMP-9在胃癌的侵袭和迁移中起着重要作用,其表达为胃癌预后独立因素,可作为胃癌预后指标。
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