Expression and significance of growth factor receptor binding protein 2-associated binding protein-1 and glioma-associated oncogene homologue-1 in pediatric medulloblastoma

Abstract

Objective To explore the expressions of growth factor receptor binding protein 2-associated bin-ding protein-1(Gab-1) and glioma-associated oncogene homologue-1(Gli-1) in pediatric medulloblastoma, and to analyze their correlation between clinical and pathological characteristics and prognosis in pediatric medulloblastoma. Methods Elivision immunohistochemistry was used to detect the expressions of Gab-1 and Gli-1 protein in tissue microarray of 40 paraffin embedded pediatric medulloblastoma specimens.Chi-square test or Fisher exact test was used to analyze the correlation between Gab-1 and Gli-1 protein expressions with gender, age, tumor location and pathological subtypes.Follow-up data were handled by using Kaplan-Meier survival analysis and Cox regression analysis. Results Positive expression ratios of Gab-1 and Gli-1 protein in 40 pediatric medulloblastoma were 35.0% and 55.0%, respectively.The positive expression rate of Gab-1 in medulloblastoma tissues had no statistical significance between different genders[male: 30.4%(7/23 cases) vs.female: 41.2%(7/17 cases)], age[ 0.05). There were statistical differences of positive expression rate of Gli-1 protein in different age groups[ 0.05). Kaplan-Meier survival analysis showed that the age, the expressions of Gab-1 and Gli-1 protein were correlated with prognosis of pediatric medulloblastoma(all P<0.05). Cox regression indicated that the age, pathological subtypes and the expression of Gli-1 protein were independent prognostic indicators in pediatric medulloblastoma(all P<0.05). Conclusion Expression of Gab-1 and Gli-1 protein is significantly correlated with the prognosis of medulloblastoma, and the positive expression is a marker of unfavorable prognosis. Key words: Growth factor receptor binding protein 2-associated binding protein-1; Glioma-associated oncogene homologue-1; Child; Medulloblastoma; Prognosis