Expression and significance of COX-2, ICAM-1 and IFN- γ in serum of rats with chronic prostatitis

Abstract

Objective To investigate the expression and significance of Cyclooxygenase-2 (COX-2), Intercellular adhesion molecular-1 (ICAM-1), Interferon-γ(IFN-γ) in serum of chronic prostatitis rats. Methods Forty-five male Wistar rats were randomly divided into the control group and the model group, which were provided by the animal research center of Jiamusi university. The model group was constructed with the model of chronic prostatitis. The expression level of COX-2 protein, ICAM-1 protein and IFN-γ protein was detected by Western blot, and the expression level of COX-2 mRNA, ICAM-1 mRNA and IFN-gamma mRNA was detected by RT-PCR. Results The expression of COX-2, ICAM-1, IFN-γ protein and mRNA in the control group and the model group were consistent. The expression level of COX-2 protein and COX-2 mRNA in the model group was significantly higher than that in the control group(P<0.05), and the expression level of ICAM-1 protein and ICAM-1 mRNA in the model group was significantly higher than that of the control group(P<0.05), and the expression level of IFN-γ protein and IFN-γ mRNA in the model group was significantly higher than that of the control group(P<0.05). The sensitivity of the three indexes was detected. Sensitivity, specificity and accuracy were all higher than those of the other two items. The sensitivity, specificity and accuracy of the three joint tests were significantly higher than those of the single test, with a statistically significant difference(P<0.05). The expression of prostaglandin increased with the increase of COX-2 expression, and the different stages of chronic prostatitis also had an effect on its secretion. Conclusions The changes of expression level of COX-2, ICAM-1 and IFN-γ are closely related to chronic prostatitis. It is very important for the diagnosis, treatment and prognosis of chronic prostatitis to monitor the changes of expression level of COX-2, ICAM-1 and IFN-γ. Key words: Prostatitis; Models, Animal; Cyclooxygenase 2; Junctional Adhesion Molecules; Interferon-gamma