Expression and significance of chemokine receptor CXC chemokine receptor-5 and its ligand CXCL13 in colon cancer

Abstract

Objective To investigate the correlation between CXC chemokine receptor-5 (CXCR5)-CXCL13 expression and clinicopathological features in colorectal carcinoma.Methods The real-time transcription polymerase chain reaction (Real-time PCR) technique was used to examine mRNA expression of CXCR5 and CXCL13 in 25 paired specimens of colorectal cancer and tissues adjacent carcinoma and 13 samples of colorecal adenoma.The immunohistochemistry staining method was used to detect the protein expression of CXCR5 and CXCL13 in 153 paired specimens of colorectal cancer and tissues adjacent carcinoma and 62 samples of colorecal adenoma.Results The mRNA and protein levels of CXCR5-CXCL13 in colorectal cancer were up-regulated than tissues adjacent carcinoma and adenoma (P ＜ 0.05).The mRNA and protein levels of CXCR5-CXCL13 in colorectal cancer correlated positively (PCR:r =0.681,P ＜ 0.01 ; IHC:r =0.196,P ＜ 0.05).The positive stain of CXCR5-CXCLI3 correlated with lymph node involved,metastasis,higher TNM stage and relapse (P ＜ 0.05).Additionally,CXCL13 positive staining correlated with poor differentiation (P ＜ 0.05).By Kaplan-Merie' s analysis,CXCR5-CXCL13 positive staining favored poor prognosis parameters,such as 5-year relapse rate (CXCR5:48.6％ vs.14.8％,CXCL13:41.5％ vs.22.7％),5-year survival rate (CXCR5:55.6％ vs.91.4％,CXCL13:61.5％ vs.84.1％),the median disease free time [CXCR5:(13.0 ± 1.3) months vs.(45.0 ± 7.8) months,CXCL13:(13.0 ± 1.3) months vs.(29.0 ± 11.2) months] and the median survival time [CXCR5:(17.0 ± 1.1) months vs.(55.0 ± 14.4) months,CXCL13:(17.0 ± 1.9) months vs.(25.0 ± 11.2)months] (P ＜0.05).Conclusion CXCR5 and CXCL13 may play a crucial role in carcinogenesis,development,metastasis and relapse of colon cancer.It can be used as prognostic markers of colon cancer in clinical practice. Key words: CXC chemokine receptor-5; CXCL13; Colorectal cancer; Metastasis; Prognosis