Expression and significance of amplified in breast cancer 1 in intrahepatic cholangiocarcinoma

Abstract

Objective To investigate the amplified in breast cancer 1 (AIB1) and epithelial mesenchymal transition (EMT) related molecular markers in intrahepatic cholangiocarcinoma (ICC) and its relationship with clinical pathology. Methods The expression of AIB1 was detected by immunohistochemistry in the tissue adjacent to carcinoma and ICC. Recation between AIB1 clinical pathology of ICC was analyzed. Results The results of immunohistochemistry showed that the expression of AIB1 protein in ICC tissue was significantly higher than that in adjacent tissues, and the expression level of N-cadherin and Vimentin in ICC tissue was significantly higher than that in adjacent tissues. There was no significant difference in AIB1 protein expression with age, sex, tumor diameter, distant metastasis and pathological typing. The lymph node metastasis of ICC cases in positive expression of AIB1 occurred was 48.71% (19/39), and lymph node metastasis of ICC in negative expression of AIB1 occurred was 24.32% (9/37), there was statistically significant (P=0.035). In addition, the lymph node metastasis of ICC cases in positive expression of N-cadherin occurred was 47.50% (19/40), and lymph node metastasis of ICC in negative expression of N-cadherin occurred was 25.00% (9/36), two with statistical difference (P=0.027). There was no significant difference in Vimentin protein expression with age, sex, tumor diameter, distant metastasis and pathological typing. Western blotting assay results showed that AIBl expression in cancer tissue with positive lymph node metastasis was significantly higher than that in cancer tissue with negative lymph node metastasis, the difference was statistically significant (P=0.034). Conclusion The expression of AIB1 in ICC is associated with ICC lymph node metastasis and epithelial mesenchymal transition, suggesting that AIB1 may promote the invasion and metastasis of ICC by inducing epithelial mesenchymal transition of ICC. Key words: Intrahepatic cholangiocarcinoma; Amplified in breast cancer 1; Epithelial mesenchymal transition; Invasion and metastasis