Abstract
The plant protein thaumatin has been expressed and secreted in Aspergillus oryzae. The thaumatin II cDNA was expresed via a Saccharomyces cerevisiae glyceraldehyde 3-phosphate dehydrogenase (GPD) promoter, and its secretion is apparently directed into the medium by the native plant signal sequence. The S. cerevisiae GPD promoter is properly regulated in A. oryzae; the addition of glucose stimulates the production of thaumatin. A 22-kDa immunoreactive protein indistinguishable from mature thaumatin is present in the culture medium. A slightly larger, 25-kDa protein is observed in cell extracts and may represent the unprocessed form of the protein. Maximum accumulation of the extracellular immunoreactive protein is observed after two days and no further production is observed.
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