Abstract
The present study examines the expression and regulation of gonadotropin‐releasing hormone (GnRH) and its receptor (GnRH‐R) mRNA levels during mouse ovarian development. A fully processed, mature GnRH mRNA together with intron‐containing primary transcripts was expressed in the immature mouse ovary as determined by Northern blot analysis and reverse transcription‐polymerase chain reaction (RT‐PCR). The size of ovarian GnRH mRNA was similar to that of hypothalamus, but its amount was much lower than that in the hypothalamus. Quantitative RT‐PCR procedure also revealed the expression of GnRH‐R mRNA in the ovary, but the estimated amount was a thous and‐fold lower than that in the pituitary gland. We also examined the regulation of ovarian GnRH and GnRH‐R mRNA levels during the follicular development induced by pregnant mare's serum gonadotropin (PMSG) and/or human chorionic gonadotropin (hCG). Ovarian luteinizing hormone receptor (LH‐R) mRNA was abruptly increased at 48 h after the PMSG administration and r...
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