Abstract
AtCASPL1D2 belongs to the non-featured protein family UPF0497. To investigate the optimal conditions for its protein expression and purification, the AtCASPL1D2 gene from wild-type Arabidopsis thaliana was cloned and constructed into the protein expression vector pET-32a. The AtCASPL1D2 gene was expressed in Escherichia coli BL21 as a His fusion protein. The His-AtCASPL1D2 fusion protein was induced and purified under the optimized culturing condition of 0.2 mM IPTG and 20 ℃ for 5 h. The study laid the foundation for subsequent experiments to analyze the activity of AtCASPL1D2 protein and to verify the interaction between the gene and the proteins.
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