Expression and prognostic value of CLIC1 in epithelial ovarian cancer.

Abstract

The clinical significance of the chloride intracellular channel 1 (CLIC1) protein in ovarian cancer is yet to be determined. The present study aimed to investigate the association between CLIC1 expression, and clinicopathological features and prognosis of patients with epithelial ovarian cancer. In this retrospective study, CLIC1 level was determined by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemical staining. The association between CLIC1 expression and clinicopathological characteristics were evaluated. Progression-free survival and overall survival were assessed by univariate, and multivariate analyses. mRNA and protein levels of CLIC1 were significantly higher in cancerous tissues than in healthy ovarian tissues (P<0.001). CLIC1 signals in epithelial ovarian cancer tissues were significantly higher than that in healthy tissues (P<0.001). CLIC1 expression was significantly higher in higher-grade tumors than in low-grade tumors (P<0.001). Moreover, overexpression of CLIC1 was associated with cisplatin resistance (P<0.001). CLIC1 expression was an independent factor that predicted shorter progression-free survival (P=0.006) and overall survival (P=0.002) for patients with epithelial ovarian cancer. These findings indicate that CLIC1 is overexpressed and is associated with poor prognosis in patients with epithelial ovarian cancer.