Expression and prediction of genes related to IGF2BP3 in gastric cancer*

Abstract

Abstract Objective Gastric cancer (GC) is one of the most prevalent cancers worldwide and is associated with high morbidity and mortality rates. The IGF2 mRNA-binding protein (IGF2BP) participates in a variety of cancers. The aim of this study was to analyze the expression of IGF2BP3 and explore the genes related to IGF2BP3 in GC. Methods Bioinformatics software was used to analyze the expression of IGF2BP1, IGF2BP2, and IGF2BP3 in tumors, and the expression of IGF2BPs in the GSE118897 dataset. Immunohistochemistry was performed to detect the protein level of IGF2BP3 in GC samples. cBioPortal was used to query gene alteration of IGF2BP3. LinkedOmics was used to identify genes related to IGF2BP3. Results Sangerbox analysis showed that the expression of all IGF2BP family members was higher in GC. cBioporta analysis showed that gene alteration of IGF2BP3 in stomach adenocarcinoma included mutation and amplificatio. LinkedOmics analysis showed that many genes were correlated with IGF2BP3, such as PLAGL2, GET4, IGF2BP1, HMGA2, CLDN6, HOXC13, SMARCA2, TMEM66, CIRBP, NFIX, SLC25A12, and CYB5D2. Conclusion In this study, we founded that IGF2BP3 was overexpressed in GC. Furthermore, this study identified potential genes related to IGF2BP3 in GC, which should be studied further.