Expression and Mechanism of KAT2A and CDK4/CDK6 in Children with Acute Leukemia

Abstract

To investigate the expression and mechanism of histone acetyltransferase 2A (KAT2A) and cyclin D1 dependent kinase 4/6 (CDK4/CDK6) in children with acute leukemia(AL). Seventy-one children with leukemia were selected from June 2017 to November 2018 and were enrolled in AL children group. 59 persons of healthy physical examination were selected and enrolled in control group. Real-time fluorescent quantification PCR chip was used to determine the expression of histone-modified genes and the expression levels of CDK4, CDK6 and CDK4/CDK6 mRNA; Western blot was used to validated KAT2A-positive expression; SPSS Pearson correlation analysis software was used to analyze the correlation between KAT2A and CDK4/CDK6 expression in children with acute leukemia. By comprehensive analysis of the existing chip data, the positive expression of KAT2A in the AL children group was 78.87%, which was higher than that in the control group (8.47%) (P<0.05). The expression level of CDK4 and CDK6 mRNA in the AL children group was higher than that in the control group (P<0.05); the expression level of CDK4/CDK6 mRNA in the AL children group was lower than that in the control group (P<0.05). The results of Western blot showed that the expression levels of KAT2A, CDK4 and CDK6 in AL children were higher than those in the control group, while the CDK4/CDK6 expression level in AL children group was lower than that in control group. The results of SPSS Pearson correlation analysis showed that the positive expression rate of KAT2A in AL children group positively correlated with expression level of CDK4 and CDK6 mRNA（r=0.673, r=0.559）, and negatively correlated with expression level of CDK4/CDK6 mRNA（r=-0.762）. The KAT2A is highly expressed in AL children, while the CDK4/CDK6 is lowly expressed in AL children, there is a significant correlation between of them, which is expected to become a new target for the treatment of acute leukemia children.