Expression and localization of the nuclear factor-kB noncanonical signaling pathway in the radial artery of diabetic patients

Abstract

Objective To observe the expression and localization of the nuclear factor-κB(NF-κB) noncanonica signaling pathway in the radial artery of the coronary artery bypass grafting(CABG)patients with diabetes and non-diabetes. Methods Samples of radial arter from 20 cases of diabetic and 20 cases of non-diabetic patients who undergone CABG were collected from June 2013 to December 2014. HE staining techniques was used to test the morphology of radial artery. Immunohistochemical techniques was then used to test the expression and localization of key factors[inhibitor of kappa B kinase α(IKKα)、P52、relB、interleukin-10(IL-10)]in the NF-κB noncanonical signaling pathway in the two groups of patients. Results The radial artery intima was significantly thickened in the diabetic patients when compared with non-diabetic patients by HE staining. And there were a lot of inflammation and foam cell infiltration in the radial artery intima of the diabetic patients. Using immunohistochemical techniques found that the expression of IKKα, P52, relB factors in diabetic group were obviously higher than non-diabetic group(4.7±0.7 vs.1.2±0.6, t=17.558; 5.1±0.7 vs.1.0±0.6, t=20.044; 4.1±0.7 vs.1.1±0.7, t=13.280; all P<0.01). In the aspect of anti-inflammatory cytokines, the expression of IL-10 factor in diabetic group was significantly higher than non-diabetic group(5.0±0.7 vs.1.1±0.6, t=19.066, P<0.01). And the four kinds of protein were mainly located in radial artery intima layer cells in diabetic patients. Conclusion Expression levels of 4 key factors in NF-κB noncanonical signaling pathway are significantly higher in radial artery intimal layer of diabetic patients than non-diabetic patient’s. And positive signals are more concentrated in the radial artery intima layer of cells. Key words: Coronary artery bypass; Radial artery; Diabetes mellitus; NF-kappaB