Abstract
Protein kinase C (PKC) is encoded by a complex of a gene family, and its multiple isoforms are expressed in various mammalian tissues. The objective of this study was to investigate the expression and localization of a PKC θ isoform in mouse testis. PKC θ displays the highest homology to PKC δ, lacks the Ca 2+-binding C2 domain and, thus, belongs to the subfamily of Ca 2+-independent PKC enzymes which also includes the δ, ε, ζ and η isoforms. We analyzed the PKC θ mKNA and protein by Northern blotting, in situ hybridization, and immunohistochemistry. In testes of normal mice, signals of PKC θ isoform expression were detected specifically in the interstitial cells of testes. The expression of PKC θ isoform was also detected in testes of germ cell-deficient W W′ mice. These results suggest that PKC θ isoform has the specific biological functions in the interstitial cells of testis.
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