Expression and Localization of Endothelin-1 and its Receptors in the Spiral Ganglion Neurons of Mouse

Abstract

Endothelin-1 (ET-1) is a peptide with various biological functions, such as vasoconstriction and cell proliferation. ET-1 was reported to be widely distributed throughout the animal body, including nervous system. The expression and localization of ET-1 and its receptors [endothelin type-A receptor (ETAR) and endothelin type-B receptor (ETBR)] in the spiral ganglion neurons have not been reported before. In this study, their presence in the mouse spiral ganglion neurons was detected at mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) technique and immunohistochemistry, respectively. RT-PCR analysis indicated that ET-1, ETAR, and ETBR genes were expressed in the mouse spiral ganglion tissues. Immunohistochemical experiments demonstrated that ET-1 and ETAR were predominantly immunoreactive in the cytoplasm, while ETBR was mainly immunostained in the nucleus of the neuron bodies. The present results suggest that ET-1 may play physiological roles in the spiral ganglion cells via ETAR and ETBR.