Abstract
Phosphoinositide-specific phospholipase C (PI-PLC) is a critical enzyme family, participating in various signal-transduction pathways in eukaryotic cells. However, little information on PI-PLC in plants is available. In this study, a PI-PLC gene, PtoPLC1 was obtained from Populus tomentosa and initial characterized. The recombinant PtoPLC1 protein expressed in vitro was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The catalysis of PIP2 by PtoPLC1 is Ca2+ dependent and the optimum concentration of Ca2+ is 1 μM. These results suggested that PtoPLC1 gene encodes a functional PI-PLC. The qReal-time PCR analysis revealed strong expression of PtoPLC1 in roots, but weak expression in stems and leaves. The gene is strongly induced under high salt, dehydration and ABA. These results suggest that PtoPLC1 might be involved in the signal-transduction pathways of high salt, dehydration and ABA responses in P. tomentosa.
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