Expression and cloning of the full-length cDNA for sorbitol-6-phosphate dehydrogenase and NAD-dependent sorbitol dehydrogenase from pear ( Pyrus pyrifolia N.)

Abstract

Sorbitol is the primary product of photosynthesis and the major translocation form of photosynthate in the Rosaceae family, which includes pear plants. Therefore, the biosynthesis and breakdown of sorbitol is an important step in the carbohydrate metabolism of pear. In sorbitol metabolism, sorbitol-6-phosphate dehydrogenase (S6PDH) and NAD-sorbitol dehydrogenase (NAD-SDH) play key roles in biosynthesis and breakdown of sorbitol, respectively. We isolated full-length cDNAs encoding S6PDH and NAD-SDH from pear ( Pyrus pyrifolia N. cv Niitaka). PyS6PDH cDNA encodes a polypeptide consisting of 311 amino acid residues. PyNAD-SDH cDNA encodes a 371 amino acid polypeptide. The PyS6PDH gene was expressed at high levels in leaves at 30 day after full bloom (DAFB), but its expression was decreased later. Conversely, the transcripts of the PyNAD- SDH gene were detected at low levels in young leaves, but increased with age. These genes may be regulated by the transcriptional level.