Expression and clinically pathological analysis of the protein CDC6 and HOXA5 in esophageal squamous cell carcinoma

Abstract

Objective To investigate the expression and clinical significance of cell division cycle 6 (CDC6) and homeobox gene A5 (HOXA5) in esophageal squamous cell carcinoma. Methods The expression of CDC6 and HOXA5 in 51 specimens esophageal squamous cell carcinoma and 27 normal specimens esophageal tissues were evaluated by immunohistochemistry. Analyzed the relationship among the expression of CDC6 and HOXA5 protein and the clinicopathologic features of esophageal squamous cell carcinoma, along with the correlation between these two proteins. Results Immunohistochemical results showed that the positive expression rate of CDC6 and HOXA5 in esophageal squamous cell carcinoma tissue were 66.7%(34/51)and 60.8%(31/51), respectively, significantly higher than those in normal esophageal tissue18.5%(5/27), 22.2%(6/27), (χ2=16.370, P=0.000; χ2=10.528, P=0.001); There were significant positive correlation in the expression of CDC6 and HOXA5 and histological type(χ2=9.031, P=0.011; χ2=7.372, P=0.000), TNM stage(χ2=10.474, P=0.015; χ2=11.667, P=0.009), and there were no correlation in the expression of CDC6 and HOXA5 and age(χ2=0.000, P=1.000; χ2=0.001, P=0.972), sex(χ2=0.049, P=0.824; χ2=0.107, P=0.743), lymph node metastasis(χ2=3.186, P=0.074; χ2=2.212, P=0.137) in esophageal squamous cell carcinoma tissues. The expression of CDC6 and HOXA5 showed a positive correlation(r=0.454, P=0.001). Conclusion The positive expression rate of CDC6 and HOXA5 in esophageal squamous cell carcinoma tissue were significantly higher than in normal esophageal tissue and close correlation with TNM stage and differentiation. High expression of CDC6 and HOXA5 may play important roles in the occurrence, development and proliferation of esophageal squamous cell carcinoma. Key words: Esophageal neoplasms; Immunohistochemistry; Cell division cycle 6; Homeobox gene A 5