Expression and clinical significance of TUBB3 gene in invasive non-Hodgkin's lymphoma

Abstract

Objective To investigate the ralations hip between the expression of TUBB3 gene and clinical characteristics,prognosis of invasive non-Hodgkin' s lymphoma,in order to provide theoretical basis for individual therapy.Methods Sixty-five tissues of invasive non-Hodgkin' s lymphoma were choosed,Taqman RT-PCR was used to detect the expression quantities of TUBB3 gene,and the relations with clinical characteristics and prognosis was analyzed.SPSS 17.0 statistical was used to analyze.Results High expression rate of TUBB3 gene in invasive non-Hodgkin' s lymphona was 15.4％ (10/65),in diffuse large B cell lymphoma (DLBCL) was 28.1％ (9/32) and in T-cell lymphoma was 3.0％ (1/33)(x2 =7.859,P =0.005).High expression of TUBB3 gene was related with gender in DLBCL (x2 =4.802,P =0.028),female rate was 77.8％ (7/9),male rate was 22.2％ (2/9) ; Overal survive (OS)rates of high expression and low expression was 55.6％ (5/9) and 91.3％ (21/23) (x2 =5.426,P =0.020).Conclusions Higher expression of TUBB3 gene is obvious in DLBCL than that in T-cell lymphoma.In the high expression of patients,female is higher than male in DLBCL; OS of low expression is higher than that of high expression.Clinical characteristics and pronosis are not obvious in T-cell lymphoma. Key words: Invasive non-Hodgkin' s lymphoma ; TUBB3 gene ; Prognosis; Reverse transcriptase polymerase chain reaction