Expression and clinical significance of TLR2 and VEGF in pancreatic cancer

Abstract

Objective To study the expression of Toll-like receptor 2(TLR2)and VEGF in pancreatic cancer and its clinical significance.Methods The levels of TLR2 and VEGF mRNA were detected in 40 cases of pancreatic cancer and corresponding adjacent tissues by real-time fluorescence quantitative PCR.The expression of TLR2 and VEGF proteins were detected in 60 cases of pancreatic cancer and corresponding adjacent tissues by immunohistochemistry.Relationship between TLR2 and VEGF with clinicopathological characteristics was analyzed.Kaplan-Meier analysis was used to analyze effect of TLR2 and VEGF protein expression on the survival of patients.Results TLR2 and VEGF mRNA were 0.75 ±0.16 and 0.82 ±0.21 in pancreatic cancer tissue,significantly higher than that in the adjacent tissue[ (0.36 ±0.12)and (0.38 ±0.15)] (P all ＜0.05).TLR2 and VEGF protein positive rate was 56.67％ and 68.33％ in pancreatic cancer tissue,significantly higher than that in the adjacent tissue (31.67％ and 36.67％)(P all ＜0.05).TLR2,VEGF protein expression has no rebationship with patient gender,age,tumor location,and the degree of differentiation,but has relationship with tumor size,lymph node,nerve metastasis and clinical staging.TLR2,VEGF-negative patients' survival time was 16.2 months and 19.5 months,significantly longer than TLR2,VEGF-positive group(10.4 months and 12.6 months,P all ＜ 0.05).Conclusions TLR2,VEGF mediates the development of pancreatic cancer,and were closely related with clinical pathological features. Key words: Toll-like receptor 2; Vascular endothelial growth factor; Pancreatic cancer