Expression and clinical significance of platelet miRNA after high tibial osteotomy

Abstract

To explore effect and significance of lateral closed high tibial osteotomy on recovery of activity of knee joint. From February to December 2018, 78 patients with knee osteoarthritis who were treated by high tibial osteotomy for the first time were selected as the observation group, including 46 males and 32 females, aged from 55 to 73 years old with an average age of (61.78±6.39) years old, the course of disease ranged from 6 to 24 months with an average of (16.52±4.25) months;according to Kellgren-Lawrence (K-L) grading, 36 patients were gradeⅡand 42 patients were grade Ⅲ. Another 20 healthy people were selected as healthy group, including 13 males and 7 females, aged from 56 to 73 years old with an average of (60.89±7.12) years old. The expression of miR-146 in peripheral blood was compared between observation group and healthy group. At the same time, patients in observation group were followed up for 6 months. The patients whose knee joint function returned to normal or improved significantly during the follow-up period were admitted as the effective group, while the patients whose knee joint function did not improve significantly or tended to aggravate were admitted as the ineffective group. The clinical data and the relative expression of serum miR-146 between effective group and ineffective group were analyzed. The relative expression of serum miR-146 in observation group after operation was significantly higher than that in healthy group (P<0.05). The results of six months follow-up showed that 15 patients (19.23%) in ineffective group and 63 patients (80.77%) in effective group. Relative expression of miR-146 and interleukin-6 (IL-6) in ineffective group were higher than that of effective group (P<0.05). ROC curve analysis and maximum Yoden index were used to calculate the truncationvalues of the maximum area under curve (AUC) of serum miR-146 and IL-6, which was 0.859 and 0.702 respectively. The truncation values of miRNA-146 and IL-6 were 0.98 (sensitivity was 86.30%, specificity was 93.20%) and 175.65 (sensitivity was 72.80%, specificity was 69.50%). The expression level of serum miR-146 showed upward trend, which could regulate the differentiation of chondrocyte. The monitoring of miR-146 after operation has reference value for predicting the recovery of bone and joint function after operation.