Expression and Clinical Significance of Plasma miR-223 in Patients with Diabetic Nephropathy.

Abstract

MicroRNA-223 (miR-223) is associated with diabetes and kidney diseases and serves as a novel marker for diagnosing diabetic kidney disease (DKD). This study was conducted to investigate the plasma expression of miR-223 and its clinical significance in type 2 diabetes (T2DM) and diabetic nephropathy (DN) patients. In this research, 20 patients with T2DM and DN, 19 patients with T2DM, and 17 healthy volunteers were finally enrolled. miR-223 expression was detected by quantitative real-time PCR (qPCR), and the diagnostic value of miR-223 in DN was further analyzed. miR-223 was downregulated in the DN group compared to that in the T2DM group (P=0.031) and the control group (P < 0.001). Pearson's correlation analysis showed a negative correlation of miR-223 levels with an albumin-creatinine ratio (ACR) (r = -0.481; P=0.044), urine β2-microglobulin (β2-MG) (r = -0.494; P=0.037), urine α1-microglobulin (α1-MG) (r = -0.537; P=0.022), creatinine (Cr) (r = -0.664; P < 0.01), cystatin C (Cyc-C) (r = -0.553; P=0.017), and glycosylated hemoglobin (HbA1c) (r = -0.761; P < 0.01). The findings of a binary regression analysis indicated that miR-223, ACR, Cr, and α1-MG were the risk factors for DN (OR: 2.019, 1.166, 1.031, and 1.031; all P < 0.05). Furthermore, miR-223 had a favorable diagnostic value for DN (AUC: 0.752; sensitivity: 0.722; specificity: 0.842) (2.5 was utilized as the diagnostic cutoff point). miR-223 was lowly expressed in DN patients, and the evaluation of miR-223 may be a good approach for diagnosing DN.