Expression and clinical significance of PIWIL2 in hilar cholangiocarcinoma tissues and in cell lines

Abstract

Objective To explore the relationship between PIWIL2 and clinicopathological characteristics and prognosis after radical resection by detecting the expression of PIWIL2 in hilar cholangiocarcinoma tissues and in cell lines.Methods The expression of PIWIL2 was detected by immunohistochemistry in 41 hilar cholangiocarcinomas and 10 control tissues,Western-blot and immunocytofluorescent were used to investigate the PIWIL2 expression in cholangiocarcinoma cell lines QBC939 and bile duct epithelial cell lines HIBEpic.Univariate and multivariate analysis of survival was performed using the Kaplan-Meier curve in hilar cholangiocarcinoma patients who bad underwent radical resection.Result The expression of PIWIL2 in hilar cholangiocarcinoma tissues and QBC939 cells were significantly higher than the control tissues and HIBEpic cells (P ＜ 0.05).The expression of PIWIL2 in poor and moderate differentiated cholangiocarcinoma was significantly higher than the well differentiated group (P ＜ 0.05).Univariate analysis showed that high expression of PIWIL2 was associated with short-survival time after radical resection (P ＜ 0.05).Multivariate analysis showed that the expression of PIWIL2 was an independent prognostic factor after radical resection of hilar cholangiocarcinoma (P ＜ 0.05).Conclusions The expression of PIWIL2 was associated with TNM stage and differentiation; PIWIL2 was an independent factor for prognosis after radical resection of hilar cholangiocarcinoma. Key words: PIWI protein, PIWIL2 ; Hilar cholangiocarcinoma; Prognosis ; Risk factors