Expression and clinical significance of PDLIM2 in bladder carcinoma

Abstract

Objective To investigate the expression of PDLIM2 mRNA in bladder urothelium carcinoma and clinical significance.To explore the values of PDLIM2 in the prediction of postoperative recurrence or progress and prognosis.Methods PDLIM2 mRNA was detected in 80 bladder cancer tissues and 10 normal bladder epithelium tissues by using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).Correlations between PDLIM2 and clinicopathological features and prognosis were investigated by statistical analyses.Results A significant down regulation of PDLIM2 mRNA expression was observed in the tumor tissues compared with the corresponding normal bladder tissues (P ＜ 0.05),the mean expression of PDLIM2 mRNA in the cancer samples was 49.8％.The expression level of PDLIM2 mRNA was negatively correlated with the tumor grade,clinical stage,number and size (P ＜ 0.05),but not with sex and age (P ＞ 0.05).Patients with low levels of PDLIM2 mRNA expression had a poor prognosis compared with those with a high expression (P ＜0.05).Univariate and multivariate logistic regression prognostic analyses revealed that PDLIM2 mRNA was an independent prognostic factor for disease-free survival in bladder cancer.Conclusion PDLIM2 is significant in the progression of bladder cancer and the status of PDLIM2 mRNA expression is a novel prognostic marker for predicting bladder cancer disease-free survival. Key words: Bladder urothelium carcinoma; PDLIM2; Reverse transcriptase-polymerase chain reaction; Real-time quantitative polymerase chain reaction ; Prognosis