Expression and clinical significance of myeloid derived suppressor cells in chronic hepatitis B patients.

Li-Rong Lu,De-Chang Li,Chao-Shuang Lin,Zhen Xu,Jing Liu,Geng-Lin Zhang

doi:10.7314/apjcp.2014.15.10.4367

Li-Rong Lu, De-Chang Li + Show 4 more

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https://doi.org/10.7314/apjcp.2014.15.10.4367

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Abstract

We here document discovery of expression profile of myeloid derived suppressor cells (MDSCs) in chronic hepatitis B (CHB) patients and changes in the course of disease. The study population was composed of 75 outpatient HBV cases and 15 healthy control cases. Peripheral blood samples were collected for separation of mononuclear cells. Levels of MDSCs labeled with Lin-DR-CD11b+CD33+ obtained from peripheral blood mononuclear cells (PBMC), were revealed to have significant differences between the CHB and other groups. They were 0.414% for health control cases and 0.226% for CHB cases (Z=-2.356, p=0.0189). It also observed that the group of HBeAg positive cases had significant difference in MDSCs/ PBMC median (X(2)=11.877, p=0.003), compared with group of HBeAg negative cases and the healthy control group. It suggested considerable MDSCs might be involved in HBeAg immune tolerance. In addition, negative correlations between MDSCs/PBMC and parameters of ALT, AST and TBil, while positive correlation between MDSCs/ PBMC and ALB parameter were found. Multiple comparisons between the four phases and health control phase again, there was a statistically sifnificant difference (X(2)=17.198, p=0.002). Taken together, these findings may provide a new immunotherapy strategy for reduced the expression levels of MDSCs in CHB patients, through induction of an autoimmune response to virus removal.

Highlights

The vaccine could reduce the incidence of Hepatitis B widely, the Hepatitis B virus (HBV) infections and invasions remain a major global health problem
The World Health Organization repotted that there were about 20 million people had been infected by HBV, in which 3.5 million converted to chronic hepatitis and there were 0.7 million chronic hepatitis B patients had died of liver failure, cirrhosis and primary hepatocelluar carcinoma caused by HBV in 2013 (Lim et al, 2014)
The 75 HBV cases were divided into three groups, which comprised with group one for 15 immune tolerance cases, group two for inactive HBsAg carrying cases and group three for untreated chronic Hepatitis B (CHB) cases, according to the hepatitis disease stage

Summary

Introduction

The vaccine could reduce the incidence of Hepatitis B widely, the Hepatitis B virus (HBV) infections and invasions remain a major global health problem. The pathogenesis of HBV persistent infection remains unclear until now. Myeloid-derived suppressor cells (MDSCs) could strongly inhibit the T cellular response, which was found as heterogeneity cell populations derived from bone marrow in the 1980s (Dmitry, 2009; Martin et al, 2012). MDSCs were first discovered in tumor tissue, which played a role in tumor metastasis, staging and immune evasion (Qu et al, 2012). It could make colorectal carcinoma peritoneal and systemic effectively metastases in peritoneum microenvironment by experiments between tumor immunized and non-immunized mice (Yu et al, 2013)

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