Abstract
BackgroundThis study sought to detect the expression and clinical significance of miR-4516 and miR-21-5p in serum of patients with colorectal cancer.MethodsBioinformatics methods were used to analyze the expression patterns of miR-4516 and miR-21-5p in colorectal cancer. A total of 80 patients with colorectal cancer, 65 patients with benign colorectal tumors and 50 healthy persons were selected. qRT-PCR was performed to detect the expression levels of serum miR-4516 and miR-21-5p before and after operation or postoperative recurrence. The correlation of miR-4516 and miR-21-5p expression levels with the clinical characteristics and prognosis of colorectal cancer was analyzed, and that with the patient’s survival was further examined by Kaplan-Meier analysis.ResultsMiR-4516 was poorly expressed in colorectal cancer in the preoperative group, and miR-21-5p was highly expressed. While in the postoperative group, miR-4516 was up-regulated, and miR-21-5p was down-regulated. The low expression of miR-4516 was shown to be related to TNM staging, invasion degree, lymph node metastasis and distant metastasis of the patients. Whereas the high expression of miR-21-5p was proved to be correlated with TNM staging and lymph node metastasis. Kaplan-Meier survival analysis showed that high expression of miR-4516 or low expression of miR-21-5p could contribute to better overall survival.ConclusionLow miR-4516 or high miR-21-5p could be used as an independent risk factor for prognosis of colorectal cancer.
Highlights
This study sought to detect the expression and clinical significance of miR-4516 and miR-21-5p in serum of patients with colorectal cancer
Studies have shown that miRNAs participate in a series of life processes acting as important regulators during tumorigenesis and development, including individual development, organogenesis, hematopoiesis, cell proliferation, differentiation and apoptosis, tumor occurrence, invasion and metastasis [6, 7]. miRNAs are available in a non-invasive manner with high sensitivity (62 to 89%) and specificity, and they can be used to identify Colorectal cancer (CRC) patients with normal persons [8]. miR-21 is one of the miRNAs found by human earlier, and it can be highly expressed in many cancer types, such as pancreatic cancer, breast cancer, lung cancer, stomach cancer and CRC [9,10,11]
We detected the expression of miR-21-5p and miR-4516 in tissues and serum of patients with CRC, colorectal adenoma (CRA) and healthy persons, analyzed the relationship between their expression levels and clinicopathological characteristics, and explored the value of miRNAs in serum in the early diagnosis of CRC
Summary
This study sought to detect the expression and clinical significance of miR-4516 and miR-21-5p in serum of patients with colorectal cancer. Studies have shown that miRNAs participate in a series of life processes acting as important regulators during tumorigenesis and development, including individual development, organogenesis, hematopoiesis, cell proliferation, differentiation and apoptosis, tumor occurrence, invasion and metastasis [6, 7]. MiRNAs are available in a non-invasive manner with high sensitivity (62 to 89%) and specificity (from 41 to 89%), and they can be used to identify CRC patients with normal persons [8]. We detected the expression of miR-21-5p and miR-4516 in tissues and serum of patients with CRC, CRA and healthy persons, analyzed the relationship between their expression levels and clinicopathological characteristics, and explored the value of miRNAs in serum in the early diagnosis of CRC
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