Expression and clinical significance of MGMT, Ki-67 and P53 in human gliomas

Abstract

Objective To detect the expression of DNA repair enzyme O6-methylguanine DNA methyltransferase (MGMT), cell proliferation-related nuclear protein (Ki-67) and P53 protein in gliomas, and investigate the relationship and clinical significance among them and gloma grade. Methods 61 cases of brain glioma specimes and 16 cases of internal decompression of brain trauma were used to detect the expression of MGMT, Ki-67 and P53 by using immunohistochemical SP method. Results MGMT protein expression (19.67%∶0, χ2=3.729, P=0.062), Ki-67 protein expression (39.34%∶0, χ2=5.722, P=0.016) and P53 protein expression (27.87%∶0, χ2=9.146, P=0.002) showed significant differences in gliomas compared to normal brain tissues, the expression of Ki-67 was significantly higher in high-grade gliomas(Ⅲ-Ⅳ) than that in low-grade gliomas (Ⅰ-Ⅱ) (14∶10, χ2=11.718, P=0.001). No significant difference was found between the MGMT and P53. Conclusion MGMT protein can be used as a biomarker in gliomas detection; Ki-67 has a positive correlation with tumor grade, and can be used as a reference indicator of pathological grade; P53 protein expression may be used as a potential target for the treatment of gliomas. Key words: Glioma; O6-methylguanine DNA methyltransferase; Cell proliferation-related nuclear protein Ki-67; Tumor suppressor protein p53