Expression and clinical implication of NLR family, pyrin domain containing-3 mRNA in juvenile rheumatold arthritis

Abstract

Objective To examine the gene expression difference of NLR family, pyrin domain containing-3 (NLRP-3) mRNA in peripheral blood of the various subtypes of juvenile rheumatold arthritis (S-JIA and oligoarticular JIA), and analyze the correlation between the expression level of NLRP-3 mRNA and the clinical features of children. Methods The peripheral blood from 28 cases of juvenile rheumatold arthritis (including 13 cases of S-JIA13 and 15 cases of oligoarticular JIA) admitted and 20 healthy children receiving the healthy examination at the same time in the First Affiliated Hospital of Zhengzhou University from May 2014 to August 2015 were investigated. The total RNA was extracted, and the expression difference of NLRP-3 mRNA in peripheral blood was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). Results In the peripheral blood, the relative expression of NLRP-3 mRNA in the S-JIA group was (0.066 7±0.004 4), higher than that in oligoarticular JIA group (0.010 7±0.001 5) and normal control group (0.021 3±0.003 2) with the difference being statistically significant (F=5.491, P=0.01). There was no statistically significant difference between the normal control group and the oligoarticular JIA group (F=1.745, P=0.105). Conclusion The increased level of NLRP-3 mRNA is one of the most crucial factors to lead to S-JIA to produce inflammatory response. Key words: Juvenile rheumatold arthritis; Juvenile idiopathic arthritis; NLR family, pyrin domain containing-3 mRNA