Abstract
The expression of parathyroid hormone (PTH) receptors during osteoblastic differentiation was studied using long-term cultures of fetal rat calvaria (RC) cells. Scatchard and cross-linking studies with [125I]-labeled [Nle8,18, Tyr34] bovine PTH (1-34) amide indicated that RC cells expressed a single class of saturable, high affinity PTH receptors with a Kd of about 1.0 nM and a molecular mass of about 76 kDa. The number of receptors increased after plating, reached a maximum on day 15 (5.01 × 104 sites/cell) and decreased thereafter (3.82 × 104 sites/cell on day 20), whereas the osteoblastic markers, such as the alkaline phosphatase activity and number of bone nodules increased progressively up to day 20. These results suggest that PTH receptor expression changes during the osteoblastic lineage and that the highest number of receptors occurs at the differentiated stage prior to osteoblast maturation.
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