Abstract
PGE2 has been implicated in abdominal aortic aneurysm (AAA) associated hypervascularization. PGE2-metabolism involves 15-hydroxyprostaglandin-dehydrogenase (15-PGDH) the expression of which in AAA is unknown. The aim of this study was to examine the expression and cell distribution of 15-PGDH in AAA. Here, we show that 15-PGDH mRNA levels were significantly higher in aorta samples from patients undergoing AAA repair than in those from healthy multiorgan donors. Consequently, the ratio of metabolized PGE2 secreted by aortic samples was significantly higher in AAA. AAA production of total PGE2 and PGE2 metabolites correlated positively with PGI2 production, while the percentage of metabolized PGE2 correlated negatively with the total amount of PGE2 and with PGI2. Transcript levels of 15-PGDH were statistically associated with leukocyte markers but did not correlate with microvascular endothelial cell markers. Immunohistochemistry revealed 15-PGDH in the areas of leukocyte infiltration in AAA samples, mainly associated with CD45-positive cells, but not in normal aorta samples. We provide new data concerning 15-PGDH expression in human AAA, showing that 15-PGDH is upregulated in AAA and mainly expressed in infiltrating leukocytes. Our data suggest that microvasculature was not involved in PGE2 catabolism, reinforcing the potential role of microvasculature derived PGE2 in AAA-associated hypervascularization.
Highlights
Abdominal aortic aneurysm (AAA) is a vascular degenerative disease with high morbidity and mortality in the aged population in industrialized countries and mortality rates associated with rupture of abdominal aortic aneurysm (AAA) are high [1]
As gender composition was different in AAA and Normal aortas (NA) populations, we tested for differences in the levels of 15-PGDH between males and females in the NA group (AAA group was 100% males) but found no statistically significant difference between genders
This study describes the expression of the 15-PGDH in human abdominal aortic aneurysm (AAA) for the first time
Summary
Abdominal aortic aneurysm (AAA) is a vascular degenerative disease with high morbidity and mortality in the aged population in industrialized countries and mortality rates associated with rupture of AAA are high [1]. AAA formation and rupture are closely accompanied by inflammation and neovascularization of the media layer both contributing to the progressive. 15-Hydroxy-Prostaglandin-Dehydrogenase in Abdominal Aortic Aneurysm study design, data collection and analysis, decision to publish, or preparation of the manuscript
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