Expression and biological function of long non-coding RNA-regulator of reprogramming in gastric cancers

Abstract

Objective To explore the expression of regulator of reprogramming (ROR) in human gastric cancer tissues and investigate the effects of ROR on biological behaviors in vitro. Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) was used to detect the expression of ROR in 50 gastric cancer tissues and its paired normal tissues. To further explore its biological function, over-expression approach was used to up ROR expression. Normal control group (NC), plasmid control group (LV5-NC) and over-expression group (LV5-ROR)were transfected to in MGC-803, SGC-7901, and the transfection efficiency was evaluated by RT-qPCR. Cell counting kit-8 (CCK-8), transwell migration and invasion assays were performed to detect the biological effects of ROR in gastric cancer cells after transfection. Results The level of ROR was significantly down-regulated in gastric cancer compared with paired normal tissues samples (P 0.05). Transwell migration assay showed that the number of cells passing the membrance in LV5-ROR (336.33±34.93, 201.67±45.35) were decreased significantly than LV5-NC(841.67±38.19, 605.25±45.00)and NC 790.58±35.68, 620.59±49.00)(P<0.01). As is also in invasion assay, we further discovered the number of cells passing the membrance in LV5-ROR (253.33±41.63, 290.56±35.36) were lower than LV5-NC (757.67±52.73, 680.56±32.93) and NC (790.35±51.73, 780.35±32.85) (P<0.01). Conclusion Our results suggested that ROR was significantly down-regulated in gastric cancer tissues. These studies indicated over-expression of ROR in gastric cancer cells could suppressed invasion and migration, while had little effect on cell proliferation. ROR could prevent carcinogenesis and progression by inhibiting migration and invasion. Key words: Gastric cancer; Long non-coding RNA; Regulator of reprogramming