Abstract
In this experiment, the Pichia Pastoris expression plasmid pPIC9K/scFv-Mmut was constructed, and the immunotoxin scFv-Mmut was successfully expressed in the eukaryotic expression system of Pichia Pastoris. The effect and mechanism of immunotoxin scFv-Mmut on inhibiting activity and inducing apoptosis of breast cancer cells were analyzed by in vitro assay. Through this experiment, it can be found that after the treatment of BT474 cells with a certain concentration of scFv-Mmut, the cell cycle will be affected and be blocked in the S phase, which proves that scFv-Mmut can induce the apoptosis of BT474 cells and has a significant anti-breast cancer effect.
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