Abstract
Expression Analysis of miRNA 164c During Rice Regeneration in Different indica Rice Genotypes
Highlights
In vitro tissue culture is widely applied for experimental breeding and biotechnological and genetic manipulation of plants in basic and applied research
Most indica rice varieties remain recalcitrant to genetic transformation and require refinement of the tissue culture protocol for the generation of embryogenic calli or regeneration of fertile plants from transformed cells
Most indica rice varieties are recalcitrant to hereditary control
Summary
In vitro tissue culture is widely applied for experimental breeding and biotechnological and genetic manipulation of plants in basic and applied research. Embryogenesis is initiated by altered development during the in vitro regeneration of plant tissues effectuated by differential expression of genes in somatic cells. A map-based cloning strategy was utilized to isolate a major quantitative trait locus (QTL) that encoded a ferredoxin-nitrite reductase, which estimated the regeneration ability in rice (Nishimura et al, 2005) This regeneration ability was enhanced by introgression of QTL (Takeuchi et al, 2000 and Kwon et al, 2001& 2002), indicating the requirement of key loci or genes. A few indica varieties have been reported to be regenerated, this ecotype is primarily recalcitrant to genetic manipulation (Ignacimuthu et al, 2000). Both rice cultivars are regenerated as follows. Additional experiments were carried out to identify the expression pattern of miRNA 164C key molecular regulator underlying the regeneration of rice plants
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