Expression analysis of G Protein-Coupled Receptors in mouse macrophages

Jane E Lattin,Matthew J Sweet,David A Hume,Tim Wiltshire,Christopher K Glass,Kaoru Saijo,John R Walker,Jie Zhang,Andrew I Su,Stuart Kellie,Kate Schroder

doi:10.1186/1745-7580-4-5

Abstract

BackgroundMonocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity. In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages to identify family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by an inflammatory stimulus, the bacterial product lipopolysaccharide (LPS).ResultsSeveral members of the P2RY family had striking expression patterns in macrophages; P2ry6 mRNA was essentially expressed in a macrophage-specific fashion, whilst P2ry1 and P2ry5 mRNA levels were strongly down-regulated by LPS. Expression of several other GPCRs was either restricted to macrophages (e.g. Gpr84) or to both macrophages and neural tissues (e.g. P2ry12, Gpr85). The GPCR repertoire expressed by bone marrow-derived macrophages and thioglycollate-elicited peritoneal macrophages had some commonality, but there were also several GPCRs preferentially expressed by either cell population.ConclusionThe constitutive or regulated expression in macrophages of several GPCRs identified in this study has not previously been described. Future studies on such GPCRs and their agonists are likely to provide important insights into macrophage biology, as well as novel inflammatory pathways that could be future targets for drug discovery.

Highlights

Monocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity
In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages and identified family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by the pro-inflammatory stimulus, bacterial LPS
The cell and tissue panel included two of the most commonly used primary mouse macrophage models; bone marrow-derived macrophages (BMM) and thioglycollateelicited peritoneal macrophages (TEPM), as well as microglia and the widely utilized macrophage-like cell line, RAW264

Summary

Introduction

Monocytes and macrophages express an extensive repertoire of G Protein-Coupled Receptors (GPCRs) that regulate inflammation and immunity. In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages to identify family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by an inflammatory stimulus, the bacterial product lipopolysaccharide (LPS). In this study we performed a systematic micro-array analysis of GPCR expression in primary mouse macrophages and identified family members that are either enriched in macrophages compared to a panel of other cell types, or are regulated by the pro-inflammatory stimulus, bacterial LPS. Some of these GPCRs are likely to provide future targets for drug discovery in inflammatory disease settings

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