Abstract
Banana is a climacteric fruit in which ethylene plays an important role in the regulation of the ripening process. Though it is the most produced fruit in Indonesia, the current post-harvest technologies for exporting this fruit are not economically friendly. Chitosan is one of economical biopolymer for edible coating which can extend fruit shelf-life. However, little study focused on the effect of chitosan coating has been done on gene expression level. In this study, the expression levels of several 1-aminocyclopropan-1-carboxylic acid oxidase (ACO) genes, which is an enzyme to convert 1-aminocyclopropan-1-carboxylic acid to ethylene in banana were analyzed on day 0, 1, 3, 5, 7, and 9 after ethylene treatment. As a result, one gene (ID: Ma01_t11540.1) had a similar expression pattern in both control and chitosan-coated bananas while the other genes (ID: Ma03_t02700.1, Ma05_t09360.1, Ma06_t02600.1, Ma10_t01130.1) showed different expression patterns. Among these genes, two genes (ID: Ma05_t09360.1, Ma10_t01130.1) were expressed higher than the other genes and the peak was observed on day 3. It was indicated that chitosan coating might activate the ethylene biosynthesis pathway in banana while it delayed fruit ripening.
Highlights
Post-harvest fruit is living organism and its commercial value decreases during the process of storage and transportation
The expression levels of several 1-aminocyclopropan-1-carboxylic acid oxidase (ACO) genes, which is an enzyme to convert 1-aminocyclopropan-1-carboxylic acid to ethylene in banana were analyzed on day 0, 1, 3, 5, 7, and 9 after ethylene treatment
It was indicated that chitosan coating might activate the ethylene biosynthesis pathway in banana while it delayed fruit ripening
Summary
Post-harvest fruit is living organism and its commercial value decreases during the process of storage and transportation. Banana (Musa acuminata) is one of the most important crop plants consumed in many countries It is a climacteric fruit and affected by respiration and ethylene production in post-harvest maturing process (Hubert and Didier 2012). The ethylene production of banana shows a sharp increase and decrease at the beginning of the climacteric period (Burg and Burg 1962; Karikari et al 1979) This physiological climacteric characteristic ofbanana fruit leads to a fast ripening and a short shelf-life. The study of ACO gene expression could suggest if chitosan coating delays banana ripening by affecting ethylene biosynthesis or not. A good understanding of working genes involved in the ethylene biosynthesis is very important to reveal the mechanism of delaying fruit ripening and it would contribute to improve the existing post-harvest technology especially for storage of banana and other fruits
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